| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1004-1 | ||||
Resumo:Biofilms are microbial communities that produce their own extracellular matrix with their function and structure. Biofilms are found in the majority sites of the human body, i.e., Candida albicans in the oral cavity. Candida albicans biofilms are composed of proteins, carbohydrates, nucleic acids and lipids. Regarding oral diseases, Candida albicans is present in some cases of dysplasia and cancer, however, this relationship is not a consensus and it is under evaluation. In this study, proteins of Candida albicans biofilms were precipitated and identified. The precipitation of proteins was performed by acetone precipitation, to maintain the activity of the enzymes within the pool. A total of 48,8 μg*mL-1 proteins were obtained. Protease activity was measured over 24 hours and quantified by EnzChek Protease C assay kit (Molecular Probes). For the identification, an ESI-TRAP MS/MS mass spectrometry was performed. 114 proteins were identified from the secretome of Candida albicans 36-h biofilm. The identification was performed by MASCOT v2.3. Of the 114 proteins, 1 was classified as non-identified and 7 as non-characterized. These proteins sequence was compared with the Candida Genome Database (CGD) with BLASTp for identification. Of the 114 proteins identified 23% were associated with the cell wall (maintenance, signal transduction, adhesion and synthesis). 14% were related to responses to environmental stimuli. The 66% of other proteins were related to other functions, such as transport and metabolic pathways for carbohydrates and lipids. The understanding of the C. albicans biofilms secretome is important due to the presence of immunogenic proteins that can trigger the fungal-host response, signalling pathways that lead to the development of chronic diseases such as cancer. Palavras-chave: Candida albicans, Biofilms, Proteomics, Secretome Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |||||